Plasmid

Part:BBa_K1391019:Design

Designed by: Jing Wei "Raymond" Liu   Group: iGEM14_MIT   (2014-10-17)


MAV1212_hEF1a_L7Ae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1085
    Illegal EcoRI site found at 2314
    Illegal EcoRI site found at 3025
    Illegal XbaI site found at 2998
    Illegal PstI site found at 730
    Illegal PstI site found at 1011
    Illegal PstI site found at 1440
    Illegal PstI site found at 1945
    Illegal PstI site found at 2986
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1085
    Illegal EcoRI site found at 2314
    Illegal EcoRI site found at 3025
    Illegal PstI site found at 730
    Illegal PstI site found at 1011
    Illegal PstI site found at 1440
    Illegal PstI site found at 1945
    Illegal PstI site found at 2986
    Illegal NotI site found at 402
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1085
    Illegal EcoRI site found at 2314
    Illegal EcoRI site found at 3025
    Illegal BglII site found at 1694
    Illegal BglII site found at 3486
    Illegal BamHI site found at 3004
    Illegal XhoI site found at 394
    Illegal XhoI site found at 2093
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1085
    Illegal EcoRI site found at 2314
    Illegal EcoRI site found at 3025
    Illegal XbaI site found at 2998
    Illegal PstI site found at 730
    Illegal PstI site found at 1011
    Illegal PstI site found at 1440
    Illegal PstI site found at 1945
    Illegal PstI site found at 2986
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1085
    Illegal EcoRI site found at 2314
    Illegal EcoRI site found at 3025
    Illegal XbaI site found at 2998
    Illegal PstI site found at 730
    Illegal PstI site found at 1011
    Illegal PstI site found at 1440
    Illegal PstI site found at 1945
    Illegal PstI site found at 2986
    Illegal NgoMIV site found at 645
    Illegal NgoMIV site found at 926
    Illegal NgoMIV site found at 1828
    Illegal AgeI site found at 774
    Illegal AgeI site found at 1206
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2769
    Illegal BsaI.rc site found at 3387


Design Notes

Constructed through LR of pENTR_hEF1a, pENTR_L7ae, and MAV1212-RFP.

Source

L7ae is of archaeal origin; hEF1a is from the human genome

References